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hexanucleotide random primers
Hexanucleotide Random Primers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+hexanucleotide+primers/Random+Primer/pmc11061733-52-24-27
Average 96 stars, based on 6510 article reviews
Hexanucleotide Random Primers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+hexanucleotide+primers/Random+Primer/pmc11061733-52-24-27
Average 96 stars, based on 6510 article reviews
hexanucleotide random primers - by Bioz Stars,
2026-09
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cDNA Library Assay:Article Title: A novel mycovirus isolated from the plant-pathogenic fungus Alternaria dianthicola. Article Snippet: Here, we describe the molecular characterization of a novel mycovirus isolated from a phytopathogenic fungus, Alternaria dianthicola, which we have named “Alternaria dianthicola dsRNA virus 1” (AdRV1).. AdRV1 has a genome of 3,014 bp that contains two non-overlapping open reading frames (ORF1 and 2) coding for a hypothetical protein and an RNA-dependent RNA polymerase (RdRp), respectively.. Based on the RdRp, AdRV1 is phylogenetically related to some unclassified dsRNA mycoviruses, including Alternaria longipes dsRNA virus 1, and shows a distant relationship to members of the family Partitiviridae. Article Title: Complete genome sequence of a novel partitivirus infecting the phytopathogenic fungus Alternaria tenuissima. Article Snippet: In the present study, a novel double-stranded RNA (dsRNA) mycovirus designated as "Alternaria tenuissima partitivirus 2" (AttPV2), was isolated from Alternaria tenuissima strain BJ-SY-1, a phytopathogenic fungus causing muskmelon leaf blight in Beijing municipality of China.. The genome of AttPV2 comprises two dsRNA segments.. The larger segment is 1829 bp in length and has a single open reading frame (ORF), potentially encoding a 65.8-kDa RNA-dependent RNA polymerase (RdRp), and the smaller segment is 1681 bp in length and also contains a single ORF, encoding a putative coat protein (CP) with a molecular mass of 56.0 kDa. Article Title: The complete genome sequence of a new mitovirus from the phytopathogenic fungus Colletotrichum higginsianum. Article Snippet: In this study, a novel mitovirus designed "Colletotrichum higginsianum mitovirus 1" (ChMV1) was isolated from the phytopathogenic fungus Colletotrichum higginsianum.. The genome of this mitovirus is 2,893 nt in length with an A + U content of 61% and contains a large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp).. A BLASTp analysis revealed that the RdRp domain of ChMV1 had 30.25% to 61.72% sequence identity to those of members of the genus Mitovirus and showed the highest degree of similarity (61.72% identity) to Botrytis cinerea mitovirus 3 (BcMV3). Construct:Article Title: A novel mycovirus isolated from the plant-pathogenic fungus Alternaria dianthicola. Article Snippet: Here, we describe the molecular characterization of a novel mycovirus isolated from a phytopathogenic fungus, Alternaria dianthicola, which we have named “Alternaria dianthicola dsRNA virus 1” (AdRV1).. AdRV1 has a genome of 3,014 bp that contains two non-overlapping open reading frames (ORF1 and 2) coding for a hypothetical protein and an RNA-dependent RNA polymerase (RdRp), respectively.. Based on the RdRp, AdRV1 is phylogenetically related to some unclassified dsRNA mycoviruses, including Alternaria longipes dsRNA virus 1, and shows a distant relationship to members of the family Partitiviridae. Article Title: Complete genome sequence of a novel partitivirus infecting the phytopathogenic fungus Alternaria tenuissima. Article Snippet: In the present study, a novel double-stranded RNA (dsRNA) mycovirus designated as "Alternaria tenuissima partitivirus 2" (AttPV2), was isolated from Alternaria tenuissima strain BJ-SY-1, a phytopathogenic fungus causing muskmelon leaf blight in Beijing municipality of China.. The genome of AttPV2 comprises two dsRNA segments.. The larger segment is 1829 bp in length and has a single open reading frame (ORF), potentially encoding a 65.8-kDa RNA-dependent RNA polymerase (RdRp), and the smaller segment is 1681 bp in length and also contains a single ORF, encoding a putative coat protein (CP) with a molecular mass of 56.0 kDa. Article Title: The complete genome sequence of a new mitovirus from the phytopathogenic fungus Colletotrichum higginsianum. Article Snippet: In this study, a novel mitovirus designed "Colletotrichum higginsianum mitovirus 1" (ChMV1) was isolated from the phytopathogenic fungus Colletotrichum higginsianum.. The genome of this mitovirus is 2,893 nt in length with an A + U content of 61% and contains a large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp).. A BLASTp analysis revealed that the RdRp domain of ChMV1 had 30.25% to 61.72% sequence identity to those of members of the genus Mitovirus and showed the highest degree of similarity (61.72% identity) to Botrytis cinerea mitovirus 3 (BcMV3). Article Title: Cloning and molecular characterization of the ORF5 gene from a PRRSV-SN strain from Southwest China. Article Snippet: 27 To monitor the genetic variation of PRRSV, the ORF5 gene of the PRRSV-SN strain found in 28 Suining City, Sichuan Province, was cloned and sequenced.. The results showed that the PRRSV-SN 29 strain was a highly pathogenic PRRSV (HP-PRRSV) variant strain with the North American (NA) 30 genotype.. Homology analysis showed that the ORF5 gene of the PRRSV-SN isolate shared 89.4% 31 (86.5%) nucleotide (amino acid) sequence similarity with the North American strain VR-2332, 32 98.8% (96%) similarity with JXA1, and 63.8% (57.7%) similarity with the European type 33 representative strain Lelystad virus. Reverse Transcription:Article Title: A novel mycovirus isolated from the plant-pathogenic fungus Alternaria dianthicola. Article Snippet: Here, we describe the molecular characterization of a novel mycovirus isolated from a phytopathogenic fungus, Alternaria dianthicola, which we have named “Alternaria dianthicola dsRNA virus 1” (AdRV1).. AdRV1 has a genome of 3,014 bp that contains two non-overlapping open reading frames (ORF1 and 2) coding for a hypothetical protein and an RNA-dependent RNA polymerase (RdRp), respectively.. Based on the RdRp, AdRV1 is phylogenetically related to some unclassified dsRNA mycoviruses, including Alternaria longipes dsRNA virus 1, and shows a distant relationship to members of the family Partitiviridae. Article Title: Complete genome sequence of a novel partitivirus infecting the phytopathogenic fungus Alternaria tenuissima. Article Snippet: In the present study, a novel double-stranded RNA (dsRNA) mycovirus designated as "Alternaria tenuissima partitivirus 2" (AttPV2), was isolated from Alternaria tenuissima strain BJ-SY-1, a phytopathogenic fungus causing muskmelon leaf blight in Beijing municipality of China.. The genome of AttPV2 comprises two dsRNA segments.. The larger segment is 1829 bp in length and has a single open reading frame (ORF), potentially encoding a 65.8-kDa RNA-dependent RNA polymerase (RdRp), and the smaller segment is 1681 bp in length and also contains a single ORF, encoding a putative coat protein (CP) with a molecular mass of 56.0 kDa. Article Title: Leishmania tarentolae as an Antigen Delivery Platform: Dendritic Cell Maturation after Infection with a Clone Engineered to Express the SARS-CoV-2 Spike Protein Article Snippet: The RNA was dissolved in RNase-free water and purified from genomic DNA with RNase-free DNase (RQ1 DNase; Promega, Madison, WI, USA). .. One microgram of RNA was reverse transcribed into first-strand cDNA in a 20 μL final volume containing 1 mM Article Title: Upregulation of inflammasome activity and increased gut permeability are associated with obesity in children and adolescents. Article Snippet: RNA was dissolved in RNase-free water and purified from genomic DNA with RNase-free DNase (RQ1 DNase; Promega, Madison, WI). .. One microgram of RNA was retrotranscribed into first-strand cDNA in a 20 μl final volume containing 1 μM Article Title: Immunomodulatory effects of pidotimod in adults with community-acquired pneumonia undergoing standard antibiotic therapy. Article Snippet: The morbidity and mortality of community-acquired pneumonia (CAP) are still elevated and two aspects seem to contribute to a worse outcome: an uncontrolled inflammatory reaction and an inadequate immune response.. Adjuvants, including corticosteroids and intravenous immunoglobulins, have been proposed to counterbalance these effects but their efficacy is only partial.. We examined the immunomodulatory activity of Pidotimod (PDT), a synthetic dipeptide molecule in adult patients hospitalized for CAP. Article Title: The complete genome sequence of a new mitovirus from the phytopathogenic fungus Colletotrichum higginsianum. Article Snippet: In this study, a novel mitovirus designed "Colletotrichum higginsianum mitovirus 1" (ChMV1) was isolated from the phytopathogenic fungus Colletotrichum higginsianum.. The genome of this mitovirus is 2,893 nt in length with an A + U content of 61% and contains a large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp).. A BLASTp analysis revealed that the RdRp domain of ChMV1 had 30.25% to 61.72% sequence identity to those of members of the genus Mitovirus and showed the highest degree of similarity (61.72% identity) to Botrytis cinerea mitovirus 3 (BcMV3). Article Title: Cloning and molecular characterization of the ORF5 gene from a PRRSV-SN strain from Southwest China. Article Snippet: 27 To monitor the genetic variation of PRRSV, the ORF5 gene of the PRRSV-SN strain found in 28 Suining City, Sichuan Province, was cloned and sequenced.. The results showed that the PRRSV-SN 29 strain was a highly pathogenic PRRSV (HP-PRRSV) variant strain with the North American (NA) 30 genotype.. Homology analysis showed that the ORF5 gene of the PRRSV-SN isolate shared 89.4% 31 (86.5%) nucleotide (amino acid) sequence similarity with the North American strain VR-2332, 32 98.8% (96%) similarity with JXA1, and 63.8% (57.7%) similarity with the European type 33 representative strain Lelystad virus. Article Title: Multiple inflammasome complexes are activated in autistic spectrum disorders. Article Snippet: Accepted Manuscript Multiple Inflammasome Complexes are Activated in Autistic Spectrum Disorders Marina Saresella, Federica Piancone, Ivana Marventano, Martina Zoppis, Ambra Hernis, Michela Zanette, Daria Trabattoni, Matteo Chiappedi, Alessandro Ghezzo, Maria Paola Canevini, Francesca la Rosa, Susanna Esposito, Mario Clerici PII: S0889-1591(16)30054-X DOI: http://dx.doi.org/10.1016/j.bbi.2016.03.009 Reference: YBRBI 2829 To appear in: Brain, Behavior, and Immunity Received Date: 16 December 2015 Revised Date: 2 March 2016 Accepted Date: 11 March 2016 Please cite this article as: Saresella, M., Piancone, F., Marventano, I., Zoppis, M., Hernis, A., Zanette, M., Trabattoni, D., Chiappedi, M., Ghezzo, A., Canevini, M.P., la Rosa, F., Esposito, S., Clerici, M., Multiple Inflammasome Complexes are Activated in Autistic Spectrum Disorders, Brain, Behavior, and Immunity (2016), doi: http:// dx.doi.org/10.1016/j.bbi.2016.03.009 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Purification:Article Title: Complete genome sequence of a novel partitivirus infecting the phytopathogenic fungus Alternaria tenuissima. Article Snippet: In the present study, a novel double-stranded RNA (dsRNA) mycovirus designated as "Alternaria tenuissima partitivirus 2" (AttPV2), was isolated from Alternaria tenuissima strain BJ-SY-1, a phytopathogenic fungus causing muskmelon leaf blight in Beijing municipality of China.. The genome of AttPV2 comprises two dsRNA segments.. The larger segment is 1829 bp in length and has a single open reading frame (ORF), potentially encoding a 65.8-kDa RNA-dependent RNA polymerase (RdRp), and the smaller segment is 1681 bp in length and also contains a single ORF, encoding a putative coat protein (CP) with a molecular mass of 56.0 kDa. Gel Extraction:Article Title: Complete genome sequence of a novel partitivirus infecting the phytopathogenic fungus Alternaria tenuissima. Article Snippet: In the present study, a novel double-stranded RNA (dsRNA) mycovirus designated as "Alternaria tenuissima partitivirus 2" (AttPV2), was isolated from Alternaria tenuissima strain BJ-SY-1, a phytopathogenic fungus causing muskmelon leaf blight in Beijing municipality of China.. The genome of AttPV2 comprises two dsRNA segments.. The larger segment is 1829 bp in length and has a single open reading frame (ORF), potentially encoding a 65.8-kDa RNA-dependent RNA polymerase (RdRp), and the smaller segment is 1681 bp in length and also contains a single ORF, encoding a putative coat protein (CP) with a molecular mass of 56.0 kDa. Virus:Article Title: Complete genome sequence of a novel partitivirus infecting the phytopathogenic fungus Alternaria tenuissima. Article Snippet: In the present study, a novel double-stranded RNA (dsRNA) mycovirus designated as "Alternaria tenuissima partitivirus 2" (AttPV2), was isolated from Alternaria tenuissima strain BJ-SY-1, a phytopathogenic fungus causing muskmelon leaf blight in Beijing municipality of China.. The genome of AttPV2 comprises two dsRNA segments.. The larger segment is 1829 bp in length and has a single open reading frame (ORF), potentially encoding a 65.8-kDa RNA-dependent RNA polymerase (RdRp), and the smaller segment is 1681 bp in length and also contains a single ORF, encoding a putative coat protein (CP) with a molecular mass of 56.0 kDa. Article Title: Leishmania tarentolae as an Antigen Delivery Platform: Dendritic Cell Maturation after Infection with a Clone Engineered to Express the SARS-CoV-2 Spike Protein Article Snippet: The RNA was dissolved in RNase-free water and purified from genomic DNA with RNase-free DNase (RQ1 DNase; Promega, Madison, WI, USA). .. One microgram of RNA was reverse transcribed into first-strand cDNA in a 20 μL final volume containing 1 mM Article Title: Upregulation of inflammasome activity and increased gut permeability are associated with obesity in children and adolescents. Article Snippet: RNA was dissolved in RNase-free water and purified from genomic DNA with RNase-free DNase (RQ1 DNase; Promega, Madison, WI). .. One microgram of RNA was retrotranscribed into first-strand cDNA in a 20 μl final volume containing 1 μM Article Title: Immunomodulatory effects of pidotimod in adults with community-acquired pneumonia undergoing standard antibiotic therapy. Article Snippet: The morbidity and mortality of community-acquired pneumonia (CAP) are still elevated and two aspects seem to contribute to a worse outcome: an uncontrolled inflammatory reaction and an inadequate immune response.. Adjuvants, including corticosteroids and intravenous immunoglobulins, have been proposed to counterbalance these effects but their efficacy is only partial.. We examined the immunomodulatory activity of Pidotimod (PDT), a synthetic dipeptide molecule in adult patients hospitalized for CAP. Article Title: Cloning and molecular characterization of the ORF5 gene from a PRRSV-SN strain from Southwest China. Article Snippet: 27 To monitor the genetic variation of PRRSV, the ORF5 gene of the PRRSV-SN strain found in 28 Suining City, Sichuan Province, was cloned and sequenced.. The results showed that the PRRSV-SN 29 strain was a highly pathogenic PRRSV (HP-PRRSV) variant strain with the North American (NA) 30 genotype.. Homology analysis showed that the ORF5 gene of the PRRSV-SN isolate shared 89.4% 31 (86.5%) nucleotide (amino acid) sequence similarity with the North American strain VR-2332, 32 98.8% (96%) similarity with JXA1, and 63.8% (57.7%) similarity with the European type 33 representative strain Lelystad virus. Article Title: Multiple inflammasome complexes are activated in autistic spectrum disorders. Article Snippet: Accepted Manuscript Multiple Inflammasome Complexes are Activated in Autistic Spectrum Disorders Marina Saresella, Federica Piancone, Ivana Marventano, Martina Zoppis, Ambra Hernis, Michela Zanette, Daria Trabattoni, Matteo Chiappedi, Alessandro Ghezzo, Maria Paola Canevini, Francesca la Rosa, Susanna Esposito, Mario Clerici PII: S0889-1591(16)30054-X DOI: http://dx.doi.org/10.1016/j.bbi.2016.03.009 Reference: YBRBI 2829 To appear in: Brain, Behavior, and Immunity Received Date: 16 December 2015 Revised Date: 2 March 2016 Accepted Date: 11 March 2016 Please cite this article as: Saresella, M., Piancone, F., Marventano, I., Zoppis, M., Hernis, A., Zanette, M., Trabattoni, D., Chiappedi, M., Ghezzo, A., Canevini, M.P., la Rosa, F., Esposito, S., Clerici, M., Multiple Inflammasome Complexes are Activated in Autistic Spectrum Disorders, Brain, Behavior, and Immunity (2016), doi: http:// dx.doi.org/10.1016/j.bbi.2016.03.009 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Polymerase Chain Reaction:Article Title: Leishmania tarentolae as an Antigen Delivery Platform: Dendritic Cell Maturation after Infection with a Clone Engineered to Express the SARS-CoV-2 Spike Protein Article Snippet: The RNA was dissolved in RNase-free water and purified from genomic DNA with RNase-free DNase (RQ1 DNase; Promega, Madison, WI, USA). .. One microgram of RNA was reverse transcribed into first-strand cDNA in a 20 μL final volume containing 1 mM Real-time Polymerase Chain Reaction:Article Title: Leishmania tarentolae as an Antigen Delivery Platform: Dendritic Cell Maturation after Infection with a Clone Engineered to Express the SARS-CoV-2 Spike Protein Article Snippet: The RNA was dissolved in RNase-free water and purified from genomic DNA with RNase-free DNase (RQ1 DNase; Promega, Madison, WI, USA). .. One microgram of RNA was reverse transcribed into first-strand cDNA in a 20 μL final volume containing 1 mM Article Title: Multiple inflammasome complexes are activated in autistic spectrum disorders. Article Snippet: Accepted Manuscript Multiple Inflammasome Complexes are Activated in Autistic Spectrum Disorders Marina Saresella, Federica Piancone, Ivana Marventano, Martina Zoppis, Ambra Hernis, Michela Zanette, Daria Trabattoni, Matteo Chiappedi, Alessandro Ghezzo, Maria Paola Canevini, Francesca la Rosa, Susanna Esposito, Mario Clerici PII: S0889-1591(16)30054-X DOI: http://dx.doi.org/10.1016/j.bbi.2016.03.009 Reference: YBRBI 2829 To appear in: Brain, Behavior, and Immunity Received Date: 16 December 2015 Revised Date: 2 March 2016 Accepted Date: 11 March 2016 Please cite this article as: Saresella, M., Piancone, F., Marventano, I., Zoppis, M., Hernis, A., Zanette, M., Trabattoni, D., Chiappedi, M., Ghezzo, A., Canevini, M.P., la Rosa, F., Esposito, S., Clerici, M., Multiple Inflammasome Complexes are Activated in Autistic Spectrum Disorders, Brain, Behavior, and Immunity (2016), doi: http:// dx.doi.org/10.1016/j.bbi.2016.03.009 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. cDNA Synthesis:Article Title: Cloning and molecular characterization of the ORF5 gene from a PRRSV-SN strain from Southwest China. Article Snippet: 27 To monitor the genetic variation of PRRSV, the ORF5 gene of the PRRSV-SN strain found in 28 Suining City, Sichuan Province, was cloned and sequenced.. The results showed that the PRRSV-SN 29 strain was a highly pathogenic PRRSV (HP-PRRSV) variant strain with the North American (NA) 30 genotype.. Homology analysis showed that the ORF5 gene of the PRRSV-SN isolate shared 89.4% 31 (86.5%) nucleotide (amino acid) sequence similarity with the North American strain VR-2332, 32 98.8% (96%) similarity with JXA1, and 63.8% (57.7%) similarity with the European type 33 representative strain Lelystad virus. Expressing:Article Title: Multiple inflammasome complexes are activated in autistic spectrum disorders. Article Snippet: Accepted Manuscript Multiple Inflammasome Complexes are Activated in Autistic Spectrum Disorders Marina Saresella, Federica Piancone, Ivana Marventano, Martina Zoppis, Ambra Hernis, Michela Zanette, Daria Trabattoni, Matteo Chiappedi, Alessandro Ghezzo, Maria Paola Canevini, Francesca la Rosa, Susanna Esposito, Mario Clerici PII: S0889-1591(16)30054-X DOI: http://dx.doi.org/10.1016/j.bbi.2016.03.009 Reference: YBRBI 2829 To appear in: Brain, Behavior, and Immunity Received Date: 16 December 2015 Revised Date: 2 March 2016 Accepted Date: 11 March 2016 Please cite this article as: Saresella, M., Piancone, F., Marventano, I., Zoppis, M., Hernis, A., Zanette, M., Trabattoni, D., Chiappedi, M., Ghezzo, A., Canevini, M.P., la Rosa, F., Esposito, S., Clerici, M., Multiple Inflammasome Complexes are Activated in Autistic Spectrum Disorders, Brain, Behavior, and Immunity (2016), doi: http:// dx.doi.org/10.1016/j.bbi.2016.03.009 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. |